In order to see what the hell is going on inside us, we have to use a microscope can help you to see through your skull. But using these microscopes requires a tedious procedure of preparing the sample by cutting and removing. It is very difficult to look at what’s inside without opening.
Traditionally we can all just analyze the surface of the bone and many other organs but we cannot get a deep analysis. As we know that biological systems are very intricately designed and are composed of fragile particles it is very difficult to go inside. Researchers use optical instruments in such a precise manner that they can converge diffracted light in such a way that it forms an image.
Now a team of researchers has made another microscope that solves the problems faced during the in-depth analysis of biological tissues. They have found a way to make an image from diffracted infrared light after it is passed through thick bone.
Seokchan Yoon and Hojun Lee said that their microscopes are capable of deep analysis of living tissues that are not possible by any other means.
While three-photon microscopy has been used for the deep analysis of the neurons of the mouse beneath its skull. But this technique is damaging to the fragile biological structures.
By combining these imaging techniques with powerful computational tools they were able to get precise and highly magnified images of mouse neurons under his skull very successfully.
They named this technique laser-scanning reflection-matrix microscopy (LS-RMM). It is based on the conventional technique of laser confocal microscopy that not only gathers the diffracted light to form an image but also check the input and output responses of light-medium interaction.
When light passes through a certain object a few of its pass in a straight path and some a deflected. Bones are so complex structures that they are capable of deflecting light.
The more the complex is the structure more amount of light is deflected traditional microscopic techniques rely on un-deflected light but this technique gathers data through scattered light.
After recording the inputs and outputs of the reflection matrix they use a technique adaptive optics that sort out the particles that defines the structure of living tissue.
LRSMM has very limited computational power and research is needed to enhance its power in order to study the complex biological structure from deep inside. This needs to be investigated and it will reveal many amazing aspects of this technology.
This study was published in nature communications.